Description
Cellecta’s NGS Prep Kit for Cellecta's dual sgRNA Libraries cloned into the pRSL10 vectors provides a protocol and reagents for the PCR amplification from genomic DNA and NGS sequencing of sgRNA sequences from biological samples screened using these libraries. The kit includes primers and protocol to amplify and sequence both genomically integrated sgRNAs of each construct. Sufficient reagents are provided to process 12-48 DNA samples (depending on the amount of DNA/sample) with unique indexing primers to multiplex up to 12 samples run in a single lane. Software included with the kit enables rapid and convenient analysis of the sequencing. The program demultiplexes indexed samples and aligns reads from the Illumina NGS FASTQ files. The output is a spreadsheet with the counts of each sgRNA in each sample